V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)

Synonyms: AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Binding Site: pSer124

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389508

Additional Information

Alternative name: AKT1

Gene ID: 207

UniProt: P31749

Immunogen: This Phospho-AKT1-S124 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human AKT1.

Isotype: Ig

Description: Other names: RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, AKT1, PKB, RAC,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-AKT1-S124 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human AKT1.

Molecular Weight: 55686 DA

Comments:

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Synonyms: AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-AKT1 Pab (ABIN389508) in A375 cell line lysate.AKT1(arrow) was detected using the purified Pab.

Phospho-Akt-S124 (ABIN389508) was used to detect Phospho-Akt in HUVEC. Human umbilical vein endothelial cells (HUVEC) were starved overnight and stimulated with either 50ng/ml of bFGF or VEGF for 5 min. Lysates were collected and SDS-PAGE was performed on a 10% gel. Data and protocol kindly provide by Dr. Eric Murphy of David Cheresh Lab, UCSD.

Western blot analysis of anti-Phospho-AKT1 Pab (ABIN389508) transiently transfected HEK-293 cell line lysate (1ug/lane). Phoshop-AKT1 pab(arrow) was detected using the purified Pab (1:1500 dilution).

Publications

Product: Bellacosa, Chan, Ahmed et al.: "Akt activation by growth factors is a multiple-step process: the role of the PH domain." in: Oncogene, Vol. 17, Issue 3, pp. 313-25, 1998 (PubMed).

Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).