BH3 Interacting Domain Death Agonist (BID) (pSer61) antibody

Antigen: BH3 Interacting Domain Death Agonist (BID)

Synonyms: FP497, MGC15319, MGC42355, AI875481, AU022477, 2700049M22Rik, BID, MGC140420, bid, si:ch211-238n5.6, xbid, fp497, DKFZp469E066

Binding Site: pSer61

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389522

Additional Information

Alternative name: Bid

Gene ID: 12122

UniProt: Q99M39

Immunogen: This Mouse Phospho-Bid-S61 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse Bid.

Isotype: Ig

Description: Other names: BH3 interacting domain death agonist,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Mouse Phospho-Bid-S61 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse Bid.

Molecular Weight: 21952 DA

Comments:

Background: Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-Mouse Phospho-Bid-S61 Pab (ABIN389522) is used in Western blot to detect Mouse Phospho-Bid-S61 in Jurkat tissue lysate

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Renshaw, Dempsey, Barnes et al.: "Three novel Bid proteins generated by alternative splicing of the human Bid gene." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2846-55, 2004 (PubMed).

Werner, Tait, de Vries et al.: "Requirement for aspartate-cleaved bid in apoptosis signaling by DNA-damaging anti-cancer regimens." in: The Journal of biological chemistry, Vol. 279, Issue 27, pp. 28771-80, 2004 (PubMed).

Gong, Choi, Franzin et al.: "Conformation of membrane-associated proapoptotic tBid." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28954-60, 2004 (PubMed).

Broaddus, Dansen, Abayasiriwardana et al.: "Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12486-93, 2005 (PubMed).

Gao, Shao, Jiang: "Essential roles of the Bcl-2 family of proteins in caspase-2-induced apoptosis." in: The Journal of biological chemistry, Vol. 280, Issue 46, pp. 38271-5, 2005 (PubMed).