Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr506) antibody

Details for Product No. ABIN389532
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Antigen
Synonyms cdc25a2, cdc25A, sb:cb317, CDC25A2, D9Ertd393e
Epitope
pThr506
(31), (30), (24), (23), (17), (15), (15), (15), (13), (11), (9), (8), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(382), (182), (179), (62), (62), (38), (12), (2), (1), (1), (1), (1)
Host
Rabbit
(356), (29), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(15), (15), (15), (9), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(312), (135), (94), (60), (57), (46), (13), (11), (10), (4), (3), (2), (2)
Pubmed 3 references available
Quantity 400 μL
Options
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Catalog No. ABIN389532
291.50 $
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Immunogen This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.
Clone RB7856
Isotype Ig
Specificity This Phospho-CDC25A-T507 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human Cdc25A.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CDC25A
Background CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms: M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A,
UniProt P30304
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.46 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.
Expiry Date 6 months
Supplier Images
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr506) antibody The anti-Phospho-CDC25A-T506 Pab (ABIN389532) is used in Western blot to detect Phospho-CDC25A-T506 in cells transfected with wild type or mutant T506A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr506) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Chow, Siu, Fung et al.: "DNA damage during the spindle-assembly checkpoint degrades CDC25A, inhibits cyclin-CDC2 complexes, and reverses cells to interphase." in: Molecular biology of the cell, Vol. 14, Issue 10, pp. 3989-4002, 2003 (PubMed).

General Chou, Yen, Lee et al.: "Pro-apoptotic role of Cdc25A: activation of cyclin B1/Cdc2 by the Cdc25A C-terminal domain." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17833-45, 2010 (PubMed).

Kasahara, Goto, Enomoto et al.: "14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage." in: The EMBO journal, 2010 (PubMed).

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