Checkpoint Kinase 1 (CHEK1) (pSer280) antibody

Antigen: Checkpoint Kinase 1 (CHEK1)

Synonyms: CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1

Binding Site: pSer280

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389542

Additional Information

Alternative name: CHK1

Gene ID: 1111

UniProt: O14757

Immunogen: This Phospho-CHK1-S280 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280region of human CHK1.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Chk1, CHEK1, CHK1,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-CHK1-S280 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280region of human CHK1.

Molecular Weight: 54420 DA

Comments:

Background: Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-Phospho-CHK1-S280 Pab (ABIN389542) is used in Western blot to detect Phospho-CHK1-S280 in, from left to right, A2058, mouse brain, Ramos, Jurkat, HL60, and Hela tissue lysates.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Ng, Lee, Ho et al.: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004 (PubMed).

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004 (PubMed).

Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).