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Checkpoint Kinase 1 (CHEK1) (pSer280) antibody

Details for Product No. ABIN389542
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Antigen
Synonyms An08g10320, AO090003000441, CHK1, chk1, C85740, Chk1, rad27, id:ibd2720, zgc:56093, CHEK1
Epitope
pSer280
(46), (42), (31), (31), (20), (14), (11), (9), (9), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(384), (129), (123), (36), (26), (25), (14), (4), (3), (2), (2), (2), (1)
Host
Rabbit
(338), (39), (12), (7)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(302), (158), (96), (56), (50), (47), (23), (19), (11), (4), (1), (1)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN389542
291.50 $
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Immunogen This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280 of human CHK1.
Clone RB7841
Isotype Ig
Specificity This Phospho-CHK1-S280 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280region of human CHK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CHK1
Background Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
Synonyms: Serine/threonine-protein kinase Chk1, CHEK1, CHK1,
Molecular Weight 54420 DA
Gene ID 1111
UniProt O14757
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.465 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody The anti-Phospho-CHK1-S280 Pab (ABIN389542) is used in Western blot to detect Phospho-CHK1-S280 in, from left to right, A2058, mouse brain, Ramos, Jurkat, HL60, and Hela tissue lysates.
anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Ng, Lee, Ho et al.: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004 (PubMed).

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004 (PubMed).

Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).

Validation Images
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