Death-Domain Associated Protein (DAXX) (pSer495) antibody

Antigen: Death-Domain Associated Protein (DAXX)

Synonyms: DAP6, EAP1, BING2, MGC126245, MGC126246, MGC150289, DAP-3, 4921514D13Rik, im:6905684, LOC100226911

Binding Site: pSer495

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389551

Additional Information

Alternative name: DAXX

Gene ID: 1616

UniProt: Q9UER7

Immunogen: This Phospho-DAXX-S495 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S495 of human DAXX.

Isotype: Ig

Description: Other names: Death domain-associated protein 6, Daxx, hDaxx, Fas death domain-associated protein, ETS1 associated protein 1, EAP1, DAXX, BING2, DAP6,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-DAXX-S495 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S495 of human DAXX.

Molecular Weight: 81373 DA

Comments:

Background: DAXX is proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. DAXX seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. This protein down-regulates basal and activated transcription, and it also seems to act as a transcriptional co-repressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. DAXX modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-DAXX Pab (ABIN389551) in T47D cell line lysate (35ug/lane). DAXX(arrow) was detected using the purified Pab (1:40 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Gostissa, Morelli, Mantovani et al.: "The transcriptional repressor hDaxx potentiates p53-dependent apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 48013-23, 2004 (PubMed).

Chang, Lin, Fang et al.: "Daxx mediates the small ubiquitin-like modifier-dependent transcriptional repression of Smad4." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10164-73, 2005 (PubMed).

Greger, Katz, Ishov et al.: "The cellular protein daxx interacts with avian sarcoma virus integrase and viral DNA to repress viral transcription." in: Journal of virology, Vol. 79, Issue 8, pp. 4610-8, 2005 (PubMed).

Cantrell, Bresnahan: "Interaction between the human cytomegalovirus UL82 gene product (pp71) and hDaxx regulates immediate-early gene expression and viral replication." in: Journal of virology, Vol. 79, Issue 12, pp. 7792-802, 2005 (PubMed).