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E2F1 antibody (E2F Transcription Factor 1) (pSer337)

Details for Product anti-E2F1 Antibody No. ABIN389553, Supplier: Log in to see
Antigen
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
Alternatives
anti-Human E2F1 antibody for Immunofluorescence
Epitope
pSer337
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Reactivity
Human
336
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Host
Rabbit
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Clonality (Clone)
Polyclonal ()
Conjugate
This E2F1 antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Supplier
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Immunogen This E2F1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S337 of human E2F1.
Clone RB7692
Isotype Ig
Specificity This Phospho-E2F1-S337 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S337 of human E2F1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Synonyms: Transcription factor E2F1, E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, E2F1, RBBP3,
Molecular Weight 46920 DA
Gene ID 1869
UniProt Q01094
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Pathways p53 Signaling, Cell Division Cycle
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.36 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (pSer337) (ABIN389553) The anti-Phospho-E2F1-S337 Pab (ABIN389553) is used in Western blot to detect Phospho...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (pSer337) (ABIN389553) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications ODonnell, Wentzel, Zeller et al.: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005 (PubMed).

Joshi, Ordonez-Ercan, Dasgupta et al.: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005 (PubMed).

Wang, Hou, Mohapatra et al.: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005 (PubMed).

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004 (PubMed).

Saberwal, Lucas, Janssen et al.: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004 (PubMed).