SYK antibody (pTyr525, pTyr526)

Catalog No. ABIN389670

Product Details anti-SYK Antibody

Target: SYK (Spleen tyrosine Kinase (SYK))

Binding Specificity: pTyr525, pTyr526

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M, Pig, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk.

Clone: RB5669

Isotype: IgG

Application Details

Application Notes: WB: 1:250. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-SYK antibody (ABIN389670)

Schweig, Yao, Beaulieu-Abdelahad, Ait-Ghezala, Mouzon, Crawford, Mullan, Paris: "Alzheimer's disease pathological lesions activate the spleen tyrosine kinase." in: Acta neuropathologica communications, Vol. 5, Issue 1, pp. 69, (2018) (PubMed).

Köhler, Fuhr, Dinekov: "Distribution of spleen tyrosine kinase and tau phosphorylated at tyrosine 18 in a mouse model of tauopathy and in the human hippocampus." in: Brain research, Vol. 1677, pp. 1-13, (2018) (PubMed).

Greenblatt, Park, Oh, Kim, Shin, Lee, Lee, Singh, Lee, Hu, Xiao, Charles, Penninger, Lotinun, Baron, Ghosh, Shim: "CHMP5 controls bone turnover rates by dampening NF-?B activity in osteoclasts." in: The Journal of experimental medicine, Vol. 212, Issue 8, pp. 1283-301, (2015) (PubMed).

Nishikawa, Takahashi, Nakamori, Hosomi, Maruyama, Miyazaki, Izumi, Matsumoto: "The identification of raft-derived tau-associated vesicles that are incorporated into immature tangles and paired helical filaments." in: Neuropathology and applied neurobiology, (2015) (PubMed).

Satoh, Tabunoki, Ishida, Yagishita, Jinnai, Futamura, Kobayashi, Toyoshima, Yoshioka, Enomoto, Arai, Saito, Arima: "Phosphorylated Syk expression is enhanced in Nasu-Hakola disease brains." in: Neuropathology : official journal of the Japanese Society of Neuropathology, Vol. 32, Issue 2, pp. 149-57, (2012) (PubMed).

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: Syk (SYK Products)

Synonyms: p72-Syk antibody, Sykb antibody, p72syk antibody, spleen associated tyrosine kinase antibody, spleen tyrosine kinase antibody, syk antibody, SYK antibody, Syk antibody

Background: Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Molecular Weight: 72066

Gene ID: 6850

NCBI Accession: NP_001128524, NP_001167638, NP_001167639, NP_003168

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling