conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibody

Antigen: conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)

Synonyms: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Ikka, Chuk1, Fbx24, Fbxo24, AI256658, MGC25325, CHUK, chuk, MGC83432, DKFZp459O053

Binding Site: pThr23

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389762

Additional Information

Alternative name: CHUK

Gene ID: 1147

UniProt: O15111

Immunogen: This Phospho-CHUK-T23 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T23 of human CHUK.

Isotype: Ig

Description: Other names: Inhibitor of nuclear factor kappa-B kinase alpha subunit, I kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, I-kappa-B kinase 1, IKK1, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NFkappaB inhibitor kinase alpha, NFKBIKA, CHU

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-CHUK-T23 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T23 of human CHUK.

Molecular Weight: 84654 DA

Comments:

Background: CHUK is a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Dot Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Dot blot analysis of Phospho-CHUK-T23 Antibody (ABIN389762) and CHUK Non Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Xiao, Fong, Sun: "Induction of p100 processing by NF-kappaB-inducing kinase involves docking IkappaB kinase alpha (IKKalpha) to p100 and IKKalpha-mediated phosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 29, pp. 30099-105, 2004 (PubMed).

Gu, Zhu, Findley et al.: "Identification and characterization of the IKKalpha promoter: positive and negative regulation by ETS-1 and p53, respectively." in: The Journal of biological chemistry, Vol. 279, Issue 50, pp. 52141-9, 2004 (PubMed).

Buss, Dörrie, Schmitz et al.: "Constitutive and interleukin-1-inducible phosphorylation of p65 NF-{kappa}B at serine 536 is mediated by multiple protein kinases including I{kappa}B kinase (IKK)-{alpha}, IKK{beta}, IKK{epsilon}, TRAF family member-associated (TANK)-binding kinase 1 (TBK" in: The Journal of biological chemistry, Vol. 279, Issue 53, pp. 55633-43, 2004 (PubMed).

Qing, Xiao: "Essential role of IkappaB kinase alpha in the constitutive processing of NF-kappaB2 p100." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 9765-8, 2005 (PubMed).