ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C) (pSer177) antibody

Antigen: ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C)

Synonyms: Apg4b, Autl1, Atg4bl, AW048066, 2510009N07Rik, APG4C, AUTL1, AUTL3, APG4-C, FLJ14867, apg4c, atg4cl, zgc:86888, wu:fc56g07, MGC148409

Binding Site: pSer177

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389776

Additional Information

Alternative name: ATG4C

Gene ID: 84938

UniProt: Q96DT6

Immunogen: This Phospho-ATG4C-S177 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 162~192 amino acids surrounding S177 of human APG4C. Patent pending.

Isotype: Ig

Description: Other names: APG4-C, APG4C, AUTL1, AUTL3, FLJ14867, APG4 autophagy 4 homolog C, AUT-like 1, cysteine endopeptidase, AUT-like 3 cysteine endopeptidase, autophagin-3, autophagy-related cysteine endopeptidase 3, autophagy-related protein 4 homolog C, cysteine protease AT

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-ATG4C-S177 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 162~192 amino acids surrounding S177 of human APG4C. Patent pending.

Molecular Weight: 52367 DA

Comments:

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This protein is a member of the autophagin protein family, and is designated as a member of the C-54 family of cysteine proteases. APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Autophagy, Phospho-specific antibodies, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of Phospho-APG4C-S177 Antibody (ABIN389776) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Mariño, Uría, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).