Regulator of G-Protein Signalling 19 (RGS19) (pTyr143) antibody

Antigen: Regulator of G-Protein Signalling 19 (RGS19)

Synonyms: GAIP, RGSGAIP, Gaip, Camki, MGC108699, RGS19

Binding Site: pTyr143

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389783

Additional Information

Alternative name: RGS19

Gene ID: 10287

UniProt: P49795

Immunogen: This Phospho-RGS19-Y143 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 128~158 amino acids surrounding Y143 of human RGS19. Patent pending.

Isotype: Ig

Description: Other names: Regulator of G-protein signaling 19, RGS19, G-alpha interacting protein, GAIP protein, GS19, GAIP, GNAI3IP

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-RGS19-Y143 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 128~158 amino acids surrounding Y143 of human RGS19. Patent pending.

Molecular Weight: 24636 DA

Comments:

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). RGS19 enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, which stimulates autophagy by favoring the GDP-bound form of Galphai3.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of Phospho-RGS19-Y143 Antibody (ABIN389783) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: De Vries, Elenko, Hubler et al.: "GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 26, pp. 15203-8, 1997 (PubMed).

de Alba, De Vries, Farquhar et al.: "Solution structure of human GAIP (Galpha interacting protein): a regulator of G protein signaling." in: Journal of molecular biology, Vol. 291, Issue 4, pp. 927-39, 1999 (PubMed).

Ogier-Denis, Pattingre, El Benna et al.: "Erk1/2-dependent phosphorylation of Galpha-interacting protein stimulates its GTPase accelerating activity and autophagy in human colon cancer cells." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39090-5, 2001 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).