ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (pSer100) antibody

Antigen: ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A)

Synonyms: APG4A, AUTL2, Apg4a, Autl2, Atg4al, AI627006, AV169859, MGC107179, Aut2a, apg4a, autl2, MGC82614, zgc:111958, F6E13.27, DKFZp459N0219

Binding Site: pSer100

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389840

Additional Information

Alternative name: ATG4A

Gene ID: 115201

UniProt: Q8WYN0

Immunogen: This Phospho-ATG4A-S100 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 85~115 amino acids surrounding S100 of human APG4A. Patent pending.

Isotype: Ig

Description: Other names: RP5-889N15.2, APG4A, AUTL2, APG4 autophagy 4 homolog A, AUT-like 2 cysteine endopeptidase, AUT-like 2, cysteine endopeptidase, autophagin 2, autophagin-2, autophagy-related cysteine endopeptidase 2, autophagy-related protein 4 homolog A, cysteine protease

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-ATG4A-S100 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 85~115 amino acids surrounding S100 of human APG4A. Patent pending.

Molecular Weight: 45378 DA

Comments:

Background: APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with hi

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Autophagy, Phospho-specific antibodies, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of Phospho-APG4A-S100 Antibody (ABIN389840) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).