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Cyclin-Dependent Kinase 1 (CDK1) (pThr14) antibody

Antigen

Cyclin-Dependent Kinase 1 (CDK1)
Synonyms CDK1, CDC28A, MGC111195, DKFZp686L20222, Cdc2, Cdc2a, p34, cdc2, MGC92032, zgc:92032, wu:fc30e01, CDC2, MGC134454, cdc-2, xcdc2, cdc2-a, cdc28a, p34cdc2, MGC53829
Binding Site
Alternatives

pThr14
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Dot Blot (Dot), ELISA
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN389866
Quantity 400 µL  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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anti-Cyclin-Dependent Kinase 1 (CDK1) (pThr14) antibody

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name CDK1
Gene ID 983
UniProt P06493
Immunogen This Phospho-CDK1-T14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T14 of human CDK1.
Isotype Ig
Description Other names: V-abl Abelson murine leukemia viral oncogene homolog 1, ABL1
Characteristics Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity This Phospho-CDK1-T14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T14 of human CDK1.
Molecular Weight 34095 DA
Comments

Background: CDK1-T14 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Apoptosis/Necrosis, Cell Cycle, Phospho-specific antibodies, Cell Structure
Restrictions For Research Use only

Publications
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Product Chow, Siu, Ho et al.: "Differential contribution of inhibitory phosphorylation of CDC2 and CDK2 for unperturbed cell cycle control and DNA integrity checkpoints." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40815-28, 2003 (PubMed).

Shapira, Ben-Izhak, Bishara et al.: "Alterations in the expression of the cell cycle regulatory protein cyclin kinase subunit 1 in colorectal carcinoma." in: Cancer, Vol. 100, Issue 8, pp. 1615-21, 2004 (PubMed).

Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Cyclin-Dependent Kinase 1 (CDK1)", type "Antibodies"
Hosts Rabbit (137), Mouse (53)
Reactivities Human (168), Mouse (Murine) (64), Rat (Rattus) (60), Cow (Bovine) (24), Dog (Canine) (19), Horse (Equine) (18), Xenopus laevis (10), Monkey (8), Chicken (3), Primate (3), Rabbit (3), Fruit Fly (Drosophila melanogaster) (2), Amphibian (1), Arabidopsis (1), Pig (Porcine) (1), Zebrafish (1)
Applications Western Blotting (WB) (151), ELISA (75), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (73), Immunofluorescence (IF) (57), Immunoprecipitation (IP) (39), Immunohistochemistry (IHC) (30), Functional Studies (Func) (10), Immunocytochemistry (ICC) (7), Immunohistochemistry (Fixed) (IHC (fx)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Flow Cytometry (FACS) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Radioimmunoassay (RIA) (1)
Conjugates Biotin (7), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
Epitopes C-Term (23), pTyr15 (10), pThr161 (9), Internal Region (5), N-Term (3), Thr14 (3), Tyr15 (3), pSer39 (3), pThr14 (3), AA 263-287 (1), Center (1), Center,Lys200 (1), Thr161 (1), Tyr19 (1), pSer, pThr (1), pThr14, pTyr15 (1), pTyr14 (1)