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Cyclin-Dependent Kinase 1 (CDK1) (pThr14) antibody
CDK1, CDC28A, MGC111195, DKFZp686L20222, Cdc2, Cdc2a, p34
Alternatives Dot Blot (Dot), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Phospho-CDK1-T14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T14 of human CDK1.|
|Description||Other names: V-abl Abelson murine leukemia viral oncogene homolog 1, ABL1|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Phospho-CDK1-T14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T14 of human CDK1.|
|Molecular Weight||34095 DA|
Background: CDK1-T14 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Cell Cycle, Phospho-specific antibodies, Cell Structure|
|Restrictions||For Research Use only|
|Dot blot analysis of anti-CDK1-pT14 Pab (ABIN389866) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.|
Chow, Siu, Ho et al.: "Differential contribution of inhibitory phosphorylation of CDC2 and CDK2 for unperturbed cell cycle control and DNA integrity checkpoints." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40815-28, 2003 (PubMed).
Shapira, Ben-Izhak, Bishara et al.: "Alterations in the expression of the cell cycle regulatory protein cyclin kinase subunit 1 in colorectal carcinoma." in: Cancer, Vol. 100, Issue 8, pp. 1615-21, 2004 (PubMed).
Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).