Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer166) antibody

Antigen: Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2)

Binding Site: pSer166

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389885

Additional Information

Alternative name: MDM2

Gene ID: 4193

UniProt: Q00987

Immunogen: This Phospho-MDM2-pS166 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S166 of human MDM2.

Isotype: Ig

Description: Other names: Ubiquitin-protein ligase E3 Mdm2, p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-MDM2-pS166 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S166 of human MDM2.

Molecular Weight: 55232 DA

Comments:

Background: MDM2 is a target of the transcription factor tumor protein p53. It is a nuclear phosphoprotein that binds to and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. It also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-MDM2-pT166 Antibody (ABIN389885) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Milne, Kampanis, Nicol et al.: "A novel site of AKT-mediated phosphorylation in the human MDM2 onco-protein." in: FEBS letters, Vol. 577, Issue 1-2, pp. 270-6, 2004 (PubMed).

Weber, Ludwig, Morrison et al.: "HDM2 phosphorylation by MAPKAP kinase 2." in: Oncogene, Vol. 24, Issue 12, pp. 1965-72, 2005 (PubMed).

Courtois-Cox, Genther Williams, Reczek et al.: "A negative feedback signaling network underlies oncogene-induced senescence." in: Cancer cell, Vol. 10, Issue 6, pp. 459-72, 2006 (PubMed).

Meng, Kohn, Pommier: "Dose-response transition from cell cycle arrest to apoptosis with selective degradation of Mdm2 and p21WAF1/CIP1 in response to the novel anticancer agent, aminoflavone (NSC 686,288)." in: Oncogene, Vol. 26, Issue 33, pp. 4806-16, 2007 (PubMed).

Singh, Colmegna, He et al.: "Synoviocyte stimulation by the LFA-1-intercellular adhesion molecule-2-Ezrin-Akt pathway in rheumatoid arthritis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 3, pp. 1971-8, 2008 (PubMed).