DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (pTyr399) antibody

Antigen: DNA (Cytosine-5)-Methyltransferase 1 (DNMT1)

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Binding Site: pTyr399

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389908

Additional Information

Alternative name: Dnmt1

Gene ID: 1786

UniProt: P26358

Immunogen: This Phospho-Dnmt1-pY399 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y399 of human Dnmt1.

Isotype: Ig

Description: Other names: DNA (cytosine-5)-methyltransferase 1, Dnmt1, MCMT, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, AIM, DNMT

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-Dnmt1-pY399 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y399 of human Dnmt1.

Molecular Weight: 183165 DA

Comments:

Background: DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities.

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot blot~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Dnmt1 Phospho-specific Pab (ABIN389908) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Dion, Lin, Hubert et al.: "Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline." in: Human molecular genetics, Vol. 17, Issue 9, pp. 1306-17, 2008 (PubMed).

Liao, Siu, Chan et al.: "Hypermethylation of RAS effector related genes and DNA methyltransferase 1 expression in endometrial carcinogenesis." in: International journal of cancer. Journal international du cancer, Vol. 123, Issue 2, pp. 296-302, 2008 (PubMed).