Caveolin 1, Caveolae Protein, 22kDa (CAV1) (pTyr14) antibody

Antigen: Caveolin 1, Caveolae Protein, 22kDa (CAV1)

Synonyms: CAV, CGL3, BSCL3, VIP21, MSTP085, Cav, Cav-1, MGC187299, CAV1, fc07c04, wu:fc07c04, cav1a, cav1b, caveolin 1, cav, vip21, mstp085, MGC107940, Caveolin-1, cav1

Binding Site: pTyr14

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389932

Additional Information

Alternative name: CAV1

Gene ID: 857

UniProt: Q03135

Immunogen: This Phospho-CAV1-pY14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding Y14 of human CAV1.

Isotype: Ig

Description: Other names: Caveolin-1, CAV1, CAV

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-CAV1-pY14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding Y14 of human CAV1.

Molecular Weight: 20472 DA

Comments:

Background: The scaffolding protein CAV1 is the main component of the caveolae plasma membranes found in most cell types. This protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The CAV1 gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-CAV1-pY14 Antibody (ABIN389932) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Schlegel, Arvan, Lisanti: "Caveolin-1 binding to endoplasmic reticulum membranes and entry into the regulated secretory pathway are regulated by serine phosphorylation. Protein sorting at the level of the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 276, Issue 6, pp. 4398-408, 2001 (PubMed).

Smith, Campos, Wandinger-Ness et al.: "Caveolin-1-dependent infectious entry of human papillomavirus type 31 in human keratinocytes proceeds to the endosomal pathway for pH-dependent uncoating." in: Journal of virology, Vol. 82, Issue 19, pp. 9505-12, 2008 (PubMed).

Zhong, Smart, Weksler et al.: "Caveolin-1 regulates human immunodeficiency virus-1 Tat-induced alterations of tight junction protein expression via modulation of the Ras signaling." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 31, pp. 7788-96, 2008 (PubMed).