Ataxin 1 (ATXN1) (pSer776) antibody

Antigen: Ataxin 1 (ATXN1)

Synonyms: ATX1, SCA1, D6S504E, ATXN1, atxn1, Atx1, Sca1, C85907, Ataxin-1, CG4547, dAtx-1, dAtx1, DmelCG4547, sca1

Binding Site: pSer776

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389975

Additional Information

Alternative name: ATXN1

Gene ID: 6310

UniProt: NP_000323

Immunogen: This Phospho-ATXN1-pS776 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 761~791 amino acids surrounding S776 of human ATXN1.

Isotype: Ig

Description: Other names: Ataxin-1, Spinocerebellar ataxia type 1 protein, ATXN1, ATX1, SCA1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-ATXN1-pS776 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 761~791 amino acids surrounding S776 of human ATXN1.

Molecular Weight: 86923 DA

Comments:

Background: The function of the ataxins is not known. The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-ATXN1-pS776 Antibody (ABIN389975) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Lim, Crespo-Barreto, Jafar-Nejad et al.: "Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1." in: Nature, Vol. 452, Issue 7188, pp. 713-8, 2008 (PubMed).