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CD34 Molecule (CD34) (pTyr330) antibody

Antigen

CD34 Molecule (CD34)

Binding Site
Alternatives

pTyr330

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Dot Blot (Dot), ELISA
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN389983
Quantity 400 µL  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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Additional Information

Alternative name CD34
Gene ID 947
UniProt P28906
Immunogen This Phospho-CD34-pY330 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 313~342 amino acids surrounding Y330 of human CD34.
Isotype Ig
Description Other names: Hematopoietic progenitor cell antigen CD34, CD34
Characteristics Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity This Phospho-CD34-pY330 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 313~342 amino acids surrounding Y330 of human CD34.
Molecular Weight 40716 DA
Comments

Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.

Application Details

Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Stem Cells, Cell Signaling, Cell Structure, Phospho-specific antibodies
Restrictions For Research Use only

Publications

Product Simmons, Satterthwaite, Tenen et al.: "Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 1, pp. 267-71, 1992 (PubMed).

Satterthwaite, Burn, Le Beau et al.: "Structure of the gene encoding CD34, a human hematopoietic stem cell antigen." in: Genomics, Vol. 12, Issue 4, pp. 788-94, 1992 (PubMed).

Alternatives

Alternatives for antigen "CD34 Molecule (CD34)", type "Antibodies"
Hosts Mouse (194), Rabbit (67), Rat (42), Goat (4), Chicken (2)
Reactivities Human (249), Mouse (Murine) (87), Rat (Rattus) (44), Dog (Canine) (30), Primate (24), Cow (Bovine) (20), Pig (Porcine) (20), Monkey (9), Rhesus Monkey (2), Sheep (Ovine) (2), Cat (Feline) (1), Chicken (1), Rabbit (1)
Applications Flow Cytometry (FACS) (198), Western Blotting (WB) (107), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (79), Immunofluorescence (IF) (53), ELISA (48), Immunoprecipitation (IP) (43), Immunohistochemistry (Frozen Sections) (IHC (fro)) (39), Immunocytochemistry (ICC) (28), Immunohistochemistry (Fixed) (IHC (fx)) (27), Immunohistochemistry (IHC) (25), Functional Studies (Func) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (2), Cell-ELISA (cELISA) (1)
Conjugates FITC (42), PE (35), Biotin (17), APC (14), RPE (6), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), PE-Dyomics 590 (2), PE-Dyomics 647 (2), Pacific Blue (2), PerCP (2), Cy5,PE (1), Cy5.5,PE (1), PE,DY647 (1), PE,Dyomics 647 (1), PE-Cy5 (1), PerCP-Cy5.5 (1)
Epitopes C-Term (5), AA 32-290 (3), Internal Region (3), Center (2), Extracellular Domain (2), N-Term (2), AA 37-293 (1), N-Term,AA 366-382 (1), pTyr330 (1)