Mitogen-Activated Protein Kinase 7 (MAPK7) (pThr218, pTyr220) antibody

Antigen: Mitogen-Activated Protein Kinase 7 (MAPK7)

Synonyms: BMK1, ERK4, ERK5, PRKM7, Erk5-T, ATMPK7, F8D23.5, F8D23_5, MAP kinase 7, MPK7

Binding Site: pThr218, pTyr220

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389992

Additional Information

Alternative name: ERK5

Gene ID: 5598

UniProt: Q13164

Immunogen: This Bi-phospho-ERK5-pT218/pY220 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 206~236 amino acids surrounding phosphorylated T218 and Y220 of human ERK5.

Isotype: Ig

Description: Other names: Extracellular signal-regulated kinase 5, Mitogen-activated protein kinase 7

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Bi-phospho-ERK5-pT218/pY220 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 206~236 amino acids surrounding phosphorylated T218 and Y220 of human ERK5.

Molecular Weight: 88636 DA

Comments:

Background: MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step 2-sites phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of Bi-phospho-ERK5-pT218/pY220 Antibody (ABIN389992) on nitrocellulose membrane. 50ng of Bi-phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Lee, Ulevitch, Han: "Primary structure of BMK1: a new mammalian map kinase." in: Biochemical and biophysical research communications, Vol. 213, Issue 2, pp. 715-24, 1995 (PubMed).

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995 (PubMed).