Spectrin alpha Chain, Brain (SPTAN1) (pSer66) antibody

Antigen: Spectrin alpha Chain, Brain (SPTAN1)

Synonyms: NEAS, FLJ44613, (ALPHA)II-SPECTRIN, MGC112229, im:7157190, wu:fa20e05, wu:fb33g08, wu:fk32a10, zgc:112229

Binding Site: pSer66

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390001

Additional Information

Alternative name: IPF

Gene ID: 3651

UniProt: P52945

Immunogen: This Phospho-IPF-pS66 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 50~80 amino acids surrounding S66 of human IPF.

Isotype: Ig

Description: Other names: Pancreas/duodenum homeobox protein 1, Insulin promoter factor 1, Islet/duodenum homeobox-1, Somatostatin-transactivating factor 1, Insulin upstream factor 1, Glucose-sensitive factor

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-IPF-pS66 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 50~80 amino acids surrounding S66 of human IPF.

Molecular Weight: 30771 DA

Comments:

Background: PDX1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-IPF-pS66 Antibody (ABIN390001) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Khoo, Griffen, Xia et al.: "Regulation of insulin gene transcription by ERK1 and ERK2 in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 32969-77, 2003 (PubMed).