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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (pThr93) antibody

Antigen

Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A)
Synonyms LC3, LC3A, MAP1ALC3, MAP1BLC3, LC3a, 1010001H21Rik, 4922501H04Rik, MGC105263, MGC69006, map1lc3a, MGC53333, zgc:77094, MAP1LC3A, MGC89867, MGC138125
Binding Site
Alternatives

pThr93
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Dot Blot (Dot), ELISA
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN390003
Quantity 400 µL  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (pThr93) antibody

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name LC3A
Gene ID 84557
UniProt Q9H492
Immunogen This Phospho-LC3A-T93 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 83~112 amino acids surrounding T93 of human APG8a.
Isotype Ig
Description Other names: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Characteristics Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity This Phospho-LC3A-T93 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 83~112 amino acids surrounding T93 of human APG8a.
Molecular Weight 14272 DA
Comments

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein is one of the light chain subunits and can associate with either MAP1A or MAP1B.

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot~~1:500
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Autophagy, Phospho-specific antibodies
Restrictions For Research Use only

Publications
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Product He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A)", type "Antibodies"
Hosts Rabbit (80), Mouse (6), Sheep (4), Goat (2)
Reactivities Human (86), Rat (Rattus) (45), Mouse (Murine) (44), Cow (Bovine) (24), Dog (Canine) (4), Chicken (2), Opossum (Didelphimorphia) (2), Pig (Porcine) (2), Cat (Feline) (1), Fish (1), Monkey (1), Rabbit (1), Xenopus laevis (1), Zebrafish (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (69), ELISA (45), Immunofluorescence (IF) (36), Immunohistochemistry (IHC) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunocytochemistry (ICC) (10), Immunoprecipitation (IP) (6), Dot Blot (DB) (5), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes pSer12 (21), N-Term (6), AA 1-30 (4), AA 1-50 (3), AA 83-112 (2), AA 97-106 (2), Internal Region (2), pSer3 (2), AA 85-115 (1), C-Term,Cleavage Site,cleaved,AA 97-120 (1), Cleaved (1), Internal Region,AA 36-65 (1), Internal Region,AA 92-121 (1), Isoform B (1), Isoform b,Internal Region,AA 1-30 (1), N-Term,AA 1-30 (1), N-Term,AA 22-51 (1), cleaved (1)