Claudin 2 (CLDN2) (pTyr195) antibody

Antigen: Claudin 2 (CLDN2)

Synonyms: AL022813, RGD1560247, CLDN2, MGC92260, zgc:92260

Binding Site: pTyr195

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390021

Additional Information

Alternative name: CLDN2

Gene ID: 9075

UniProt: NP_065117

Immunogen: This Phospho-CLDN2-pY195 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 180~210 amino acids surrounding Y195 of human CLDN2.

Isotype: Ig

Description: Other names: claudin 2, SP82, CLD2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-CLDN2-pY195 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 180~210 amino acids surrounding Y195 of human CLDN2.

Molecular Weight: 24418 DA

Comments:

Background: Members of the claudin protein family, such as CLDN2, are expressed in an organ-specific manner and regulate the tissue-specific physiologic properties of tight junctions.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-CLDN2-pY195 Antibody (ABIN390021) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Furuse, Fujita, Hiiragi et al.: "Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin." in: The Journal of cell biology, Vol. 141, Issue 7, pp. 1539-50, 1998 (PubMed).

Morita, Furuse, Fujimoto et al.: "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 2, pp. 511-6, 1999 (PubMed).