Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180) antibody

Antigen: Mitogen-Activated Protein Kinase 14 (MAPK14)

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Binding Site: pThr180

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390025

Additional Information

Alternative name: p38 MAPK

Gene ID: 1432

UniProt: Q16539

Immunogen: This Phospho-p38 MAPK-pT180 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 168~197 amino acids surrounding T180 of human MAPK14.

Isotype: Ig

Description: Other names: Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38alpha, MAP kinase p38alpha, Cytokine suppressive anti-inflammatory drug binding protein, CSAID binding protein, CSBP, MAX-interacting protein 2, MAP kinase MXI2, SAPK2A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-p38 MAPK-pT180 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 168~197 amino acids surrounding T180 of human MAPK14.

Molecular Weight: 41293 DA

Comments:

Background: MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-p38 MAPK-T180 Antibody (ABIN390025) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Dean, Sarsfield, Tsounakou et al.: "p38 Mitogen-activated protein kinase stabilizes mRNAs that contain cyclooxygenase-2 and tumor necrosis factor AU-rich elements by inhibiting deadenylation." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39470-6, 2003 (PubMed).

Cheung, Campbell, Nebreda et al.: "Feedback control of the protein kinase TAK1 by SAPK2a/p38alpha." in: The EMBO journal, Vol. 22, Issue 21, pp. 5793-805, 2003 (PubMed).