Lin-28 Homolog A (C. Elegans) (LIN28A) (pSer86) antibody

Antigen: Lin-28 Homolog A (C. Elegans) (LIN28A)

Synonyms: CSDD1, LIN28, LIN-28, ZCCHC1, FLJ12457, Lin28, Tex17, Lin-28, Gm10299, AL024421, ENSMUSG00000070700, csdd1, lin-28, lin28a, zcchc1, lin28, MGC55584, sb:cb650, zgc:55584, RGD1566408, MGC97527

Binding Site: pSer86

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390036

Additional Information

Alternative name: LIN28

Gene ID: 79727

UniProt: Q9H9Z2

Immunogen: This Phospho-LIN28-S86 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 70~99 amino acids surrounding S86 of human LIN28.

Isotype: Ig

Description: Other names: Lin-28 homolog A, Zinc finger CCHC domain-containing protein 1, LIN28, CSDD1, LIN28A, ZCCHC1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-LIN28-S86 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 70~99 amino acids surrounding S86 of human LIN28.

Molecular Weight: 22743 DA

Comments:

Background: LIN28A acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. It is essential for skeletal muscle differentiation through the translational up-regulation of IGF2 expression.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Stem Cells, Phospho-specific antibodies, Signaling

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-LIN28-S86 Antibody (ABIN390036) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Piskounova, Viswanathan, Janas et al.: "Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein Lin28." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21310-4, 2008 (PubMed).