ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 6 (ABCB6) (C-Term) antibody

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 6 (ABCB6)

Synonyms: ABC, PRP, umat, ABC14, MTABC3, EST45597, FLJ22414, 1200005B17Rik, MGC93242, ABCB6, ABCA6, abcb6, MGC145484

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390061

Additional Information

Alternative name: ABCB6

Gene ID: 10058

UniProt: Q9NP58

Immunogen: This ABCB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 813~842 amino acids from the C-terminal region of human ABCB6.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ABCB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 813~842 amino acids from the C-terminal region of human ABCB6.

Molecular Weight: 93885 DA

Comments:

Background: The membrane-associated protein ABCB6 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This half-transporter likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

The anti-ABCB6 C-term Pab (ABIN390061) is used in Western blot to detect ABCB6 in HL60 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Allikmets, Gerrard, Hutchinson et al.: "Characterization of the human ABC superfamily: isolation and mapping of 21 new genes using the expressed sequence tags database." in: Human molecular genetics, Vol. 5, Issue 10, pp. 1649-55, 1997 (PubMed).

Furuya, Bradley, Sun et al.: "Identification of a new P-glycoprotein-like ATP-binding cassette transporter gene that is overexpressed during hepatocarcinogenesis." in: Cancer research, Vol. 57, Issue 17, pp. 3708-16, 1997 (PubMed).

Mitsuhashi, Miki, Senbongi et al.: "MTABC3, a novel mitochondrial ATP-binding cassette protein involved in iron homeostasis." in: The Journal of biological chemistry, Vol. 275, Issue 23, pp. 17536-40, 2000 (PubMed).