ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 9 (ABCB9) (C-Term) antibody

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 9 (ABCB9)

Synonyms: TAPL, KIAA1520, EST122234, mKIAA1520, Tapl, ABCB9, LOC570148

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390063

Additional Information

Alternative name: ABCB9

Gene ID: 23457

UniProt: NP_062571

Immunogen: This ABCB9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737~766 amino acids from the C-terminal region of human ABCB9.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ABCB9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737~766 amino acids from the C-terminal region of human ABCB9.

Molecular Weight: 84474 DA

Comments:

Background: The membrane-associated protein ABCB9 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined, however, this protein may play a role in lysosomes. Alternative splicing of this gene results in three known products which are likely to have different substrate specifications.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Immunohistochemistry~~1:50~100 Western Blot ~~ 1:50~200

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ABCB9 Antibody (C-term) (ABIN390063) in T47D cell line lysates (35ug/lane). ABCB9(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Allikmets, Gerrard, Hutchinson et al.: "Characterization of the human ABC superfamily: isolation and mapping of 21 new genes using the expressed sequence tags database." in: Human molecular genetics, Vol. 5, Issue 10, pp. 1649-55, 1997 (PubMed).

Zhang, Zhang, Liu et al.: "Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23287-94, 2000 (PubMed).

Kobayashi, Hori, Suita et al.: "Gene organization of human transporter associated with antigen processing-like (TAPL, ABCB9): analysis of alternative splicing variants and promoter activity." in: Biochemical and biophysical research communications, Vol. 309, Issue 4, pp. 815-22, 2003 (PubMed).