DNA Methyltransferase 1-Associated Protein 1 (DMAP1) (C-Term) antibody

Antigen: DNA Methyltransferase 1-Associated Protein 1 (DMAP1)

Synonyms: EAF2, SWC4, MEAF2, DNMAP1, DNMTAP1, FLJ11543, KIAA1425, DKFZp686L09142, Dmtap1, mKIAA1425, 1500016M21Rik, MGC109435, MGC55593, zgc:55593, T8I13.5

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390081

Additional Information

Alternative name: DMAP1

Gene ID: 55929

UniProt: NP_061973

Immunogen: This DMAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434~463 amino acids from the C-terminal region of human DMAP1.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DMAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434~463 amino acids from the C-terminal region of human DMAP1.

Molecular Weight: 52992 DA

Comments:

Background: DNA methylation can help to regulate transcriptional silencing via repressive transcription complexes, which include methyl-CpG-binding domain proteins and histone deacetylases (HDACs) DNMT1, the core enzyme for mammalian DNA methylation, can also establish a repressive transcription complex consisting of DNMT1, HDAC2, and a third protein, termed DMAP1 for 'DNMT1-associated protein.' The 467-amino acid DMAP1 protein shares approximately 98% amino acid sequence homology with the mouse protein. DMAP1 interacts directly with the N-terminal region of DNMT1, and DMAP1 can repress transcription independently of histone deacetylase activity. DNMT1, HDAC2, and DMAP1 form a complex in vivo, and DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1exists throughout the S phase, HDAC2 joins DNMT1 and DMAP1 only during late S phase. This provides a regulated means to deacetylate heterochromatin following replication.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-DMAP1 C-term Pab (ABIN390081) is used in Western blot to detect DMAP1 in HL60 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Rountree, Bachman, Baylin: "DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci." in: Nature genetics, Vol. 25, Issue 3, pp. 269-77, 2000 (PubMed).