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MLL3 (AA 4345-4374), (C-Term) antibody

Details for Product No. ABIN390123, Supplier: Log in to see
  • E330008K23Rik
  • HALR
  • mKIAA1506
  • Mll3
  • MLL3
AA 4345-4374, C-Term
Clonality (Clone)
Polyclonal ()
Western Blotting (WB)
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Immunogen This MLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4345-4374 AA from the C-terminal region of human MLL3.
Clone RB1921
Isotype Ig
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL family, including MLL3. The MLL SET domain is a histone H3 Lys4 (K4)-specific methyltransferase whose activity is stimulated with acetylated H3 peptides. MLL3 maps to 7q36, a chromosome region frequently deleted in myeloid leukaemia. The deduced MLL3 4,911-amino acid protein is more closely related to MLL2 than to MLL1 or MLL4. MLL3 has 6 plant homeodomain (PHD) fingers preceded by a cys-rich ZNF1 domain in its N terminus, a high mobility group (HMG) box, an ATPase alpha-beta signature, and a leucine zipper motif in its central region, and 2 C-terminal FY (phe-tyr) motifs and the SET domain preceded by a ZNF2 domain in its C terminus. The predicted protein also contains several putative nuclear localization motifs. Isoform II lacks ZNF1 and the PHD.

Synonyms: KIAA1506, Myeloid/lymphoid, Mixed-lineage leukemia protein 3 homolog, Histone-lysine N-methyltransferase, H3 lysine-4 specific MLL3, Homologous to ALR protein, KMT2C
Molecular Weight 541307 Da
Gene ID 58508
UniProt Q8NEZ4
Research Area Signaling, Protein Modifications, Cancer, Chromatin
Application Notes Suggested dilutions:
WB = 1:1000
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-MLL3 (AA 4345-4374), (C-Term) antibody (ABIN390123) Western blot analysis of MLL3 Antibody (C-term) (ABIN390123) in HEK 293 cell line lys...
Product cited in: Hussain, Bhan, Ansari, Deb, Bobzean, Perrotti, Mandal: "Bisphenol-A induces expression of HOXC6, an estrogen-regulated homeobox-containing gene associated with breast cancer." in: Biochimica et biophysica acta, Vol. 1849, Issue 6, pp. 697-708, 2015

Bhan, Hussain, Ansari, Bobzean, Perrotti, Mandal: "Bisphenol-A and diethylstilbestrol exposure induces the expression of breast cancer associated long noncoding RNA HOTAIR in vitro and in vivo." in: The Journal of steroid biochemistry and molecular biology, Vol. 141, pp. 160-70, 2014

Bhan, Hussain, Ansari, Kasiri, Bashyal, Mandal: "Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally induced by estradiol." in: Journal of molecular biology, Vol. 425, Issue 19, pp. 3707-22, 2013

Ansari, Hussain, Kasiri, Mandal: "HOXC10 is overexpressed in breast cancer and transcriptionally regulated by estrogen via involvement of histone methylases MLL3 and MLL4." in: Journal of molecular endocrinology, Vol. 48, Issue 1, pp. 61-75, 2012

Ansari, Hussain, Shrestha, Kasiri, Mandal: "HOXC6 Is transcriptionally regulated via coordination of MLL histone methylase and estrogen receptor in an estrogen environment." in: Journal of molecular biology, Vol. 411, Issue 2, pp. 334-49, 2011

Background publications Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003

Hillier, Fulton, Fulton, Graves, Pepin, Wagner-McPherson, Layman, Maas, Jaeger, Walker, Wylie, Sekhon, Becker, OLaughlin, Schaller, Fewell, Delehaunty, Miner, Nash, Cordes, Du, Sun, Edwards et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003