MLL3 (C-Term) antibody

Antigen: MLL3

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390123

Additional Information

Gene ID: 58508

UniProt: Q8NEZ4

Immunogen: This MLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3471~3501 amino acids from the C-terminal region of human MLL3.

Isotype: Ig

Description: Other names: KIAA1506, Myeloid/lymphoid, Mixed-lineage leukemia protein 3 homolog, Histone-lysine N-methyltransferase, H3 lysine-4 specific MLL3, Homologous to ALR protein, KMT2C

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3471~3501 amino acids from the C-terminal region of human MLL3.

Molecular Weight: 541307 DA

Comments:

Background: The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL family, including MLL3. The MLL SET domain is a histone H3 Lys4 (K4)-specific methyltransferase whose activity is stimulated with acetylated H3 peptides. MLL3 maps to 7q36, a chromosome region frequently deleted in myeloid leukaemia. The deduced MLL3 4,911-amino acid protein is more closely related to MLL2 than to MLL1 or MLL4. MLL3 has 6 plant homeodomain (PHD) fingers preceded by a cys-rich ZNF1 domain in its N terminus, a high mobility group (HMG) box, an ATPase alpha-beta signature, and a leucine zipper motif in its central region, and 2 C-terminal FY (phe-tyr) motifs and the SET domain preceded by a ZNF2 domain in its C terminus. The predicted protein also contains several putative nuclear localization motifs. Isoform II lacks ZNF1 and the PHD.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:500~1000

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Chromatin

Restrictions: For Research Use only

Images

Western blot analysis of MLL3 Antibody (C-term) (ABIN390123) in HEK 293 cell line lysates. MLL3 (arrow) was detected using the purified Pab (1:1000 dilution).

Publications

Product: Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).