Matrix Metallopeptidase 26 (MMP26) (N-Term) antibody

Antigen: Matrix Metallopeptidase 26 (MMP26)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390147

Additional Information

Alternative name: MMP26

Gene ID: 56547

UniProt: NP_068573

Immunogen: This MMP26 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP26.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP26 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP26.

Molecular Weight: 29708 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP26 may hydrolyze collagen type IV, fibronectin, fibrinogen, beta-casein, type I gelatin and alpha-1 proteinase inhibitor. It is also able to activates progelatinase B. MMP26 is expressed specifically in uterus and placenta. Is also widely expressed in malignant tumors from different sources as well as in diverse tumor cell lines.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

MMP26-T42 (ABIN390147) western blot analysis in HL-60 cell line lysates (35ug/lane). This demonstrates the MMP26 antibody detected the MMP26 protein (arrow).

Publications

Product: Park, Ni, Gerkema et al.: "Identification and characterization of human endometase (Matrix metalloproteinase-26) from endometrial tumor." in: The Journal of biological chemistry, Vol. 275, Issue 27, pp. 20540-4, 2000 (PubMed).

de Coignac, Elson, Delneste et al.: "Cloning of MMP-26. A novel matrilysin-like proteinase." in: European journal of biochemistry / FEBS, Vol. 267, Issue 11, pp. 3323-9, 2000 (PubMed).

Uría, López-Otín: "Matrilysin-2, a new matrix metalloproteinase expressed in human tumors and showing the minimal domain organization required for secretion, latency, and activity." in: Cancer research, Vol. 60, Issue 17, pp. 4745-51, 2000 (PubMed).