|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Sirtuin 1 (SIRT1) (Center) antibody
|Synonyms||SIR2L1, SIRT1, SIR2, sirtuin 1, SRT1|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This SIRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414~443 amino acids from the Center region of human SIRT1.|
|Description||Other names: NAD-dependent deacetylase sirtuin-1, hSIRT1, SIR2-like protein 1, SIR2L1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SIRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414~443 amino acids from the Center region of human SIRT1.|
|Molecular Weight||81680 DA|
Background: SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Protein Modifications|
|Restrictions||For Research Use only|
Frye: "Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity." in: Biochemical and biophysical research communications, Vol. 260, Issue 1, pp. 273-9, 1999 (PubMed).
Frye: "Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins." in: Biochemical and biophysical research communications, Vol. 273, Issue 2, pp. 793-8, 2000 (PubMed).
Langley, Pearson, Faretta et al.: "Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence." in: The EMBO journal, Vol. 21, Issue 10, pp. 2383-96, 2002 (PubMed).
Bitterman, Anderson, Cohen et al.: "Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45099-107, 2002 (PubMed).
Takata, Ishikawa: "Human Sir2-related protein SIRT1 associates with the bHLH repressors HES1 and HEY2 and is involved in HES1- and HEY2-mediated transcriptional repression." in: Biochemical and biophysical research communications, Vol. 301, Issue 1, pp. 250-7, 2003 (PubMed).
|Hosts||Rabbit (71), Mouse (11), Chicken (3), Goat (3)|
|Reactivities||Human (64), Horse (Equine) (36), Mouse (Murine) (34), Rat (Rattus) (27), Dog (Canine) (26), Pig (Porcine) (24), Chicken (19), Cow (Bovine) (19), Monkey (2), Xenopus laevis (1)|
|Applications||Immunofluorescence (IF) (48), Western Blotting (WB) (44), ELISA (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (2), Immunocytochemistry (ICC) (1)|
|Conjugates||Biotin (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)|
|Epitopes||C-Term (5), N-Term (5), pSer27 (4), pSer47 (4), AA 577-590 (2), AA 33-55 (1), AA 551-599 (1), AA 646-660 (1), Center (1), pSer171 (1), pSer257 (1)|