Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (N-Term) antibody

Antigen: Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16)

Synonyms: LRRGT00109, RGD1306243, PARP16, MGC142706, C15orf30, FLJ20509, FLJ25281, C79952, BC055447, MGC65335, MGC77744

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390268

Additional Information

Alternative name: Parp16

Gene ID: 214424

UniProt: Q7TMM8

Immunogen: This Parp16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125~154 amino acids from the N-terminal region of mouse PARP16.

Isotype: Ig

Description: Other names: Poly [ADP-ribose] polymerase 16, EC 2.4.2.30, PARP-16

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Parp16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125~154 amino acids from the N-terminal region of mouse PARP16.

Molecular Weight: 36764 DA

Comments:

Background: Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Translation Factors, Cell Cycle, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of Parp16 (arrow) using rabbit polyclonal Parp16 Antibody (N-term) (ABIN390268). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Parp16 gene (Lane 2) (Origene Technologies).

Parp16 Antibody (N-term) (ABIN390268) western blot analysis in WiDr cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the Parp16 antibody detected the Parp16 protein (arrow).

Publications

Product: Amé, Spenlehauer, de Murcia: "The PARP superfamily." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 8, pp. 882-93, 2004 (PubMed).