Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10) (C-Term,Pro222) antibody

Antigen: Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10)

Synonyms: ABAD, CAMR, ERAB, HCD2, MHBD, HADH2, MRPP2, MRX17, MRX31, SCHAD, MRXS10, SDR5C1, 17b-HSD10, DUPXp11.22, HSD17, SDR9C2, EDH17B2, HSD17B10, MGC134122, hadh2, im:6899159, wu:fj61f03, zgc:101605, Hadh2

Binding Site: C-Term,Pro222

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390287

Additional Information

Alternative name: HADH2

Gene ID: 3028

UniProt: Q6IBS9

Immunogen: This HADH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214~243 amino acids from the C-terminal region of human ABAD.

Isotype: Ig

Description: Other names: hydroxyacyl-Coenzyme A dehydrogenase, type II isoform 1, 3-hydroxyacyl-CoA dehydrogenase type II, Type II HADH, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, Endoplasmic reticulum-associated amyloid beta-peptide binding protein, Short-chain type dehydrogen

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HADH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214~243 amino acids from the C-terminal region of human ABAD.

Molecular Weight: 26923 DA

Comments:

Background: Amyloid b-peptide-binding alcohol dehydrogenase (ABAD) is a member of the family of short chain dehydrogenase/reductases, unique among this family, it binds amyloid b-peptide and exhibits enzymatic activity toward a wide variety of substrates including linear alcohols. In an amyloid beta-abundant environment, ABAD appears to trigger cell stress induced by the amyloid peptide.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of HADH2 Antibody (C-term)(ABIN390287) in Hela,CEM,NCI-H460 cell line lysates and mouse stomach tissues lysates(35ug/lane).HADH2(arrow) was detected using the purified Pab.

Western blot analysis of HADH2 (arrow) using rabbit polyclonal HADH2 Antibody (C-term) (ABIN390287). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HADH2 gene (Lane 2) (Origene Technologies).

HADH2 Antibody (C-term) (ABIN390287) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HADH2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: He, Merz, Mehta et al.: "Human brain short chain L-3-hydroxyacyl coenzyme A dehydrogenase is a single-domain multifunctional enzyme. Characterization of a novel 17beta-hydroxysteroid dehydrogenase." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 15014-9, 1999 (PubMed).

Takuma, Yao, Huang et al.: "ABAD enhances Abeta-induced cell stress via mitochondrial dysfunction." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 6, pp. 597-8, 2005 (PubMed).