C-rel (NFkB) (Center) antibody

Antigen: C-rel (NFkB)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390314

Additional Information

Gene ID: 5966

UniProt: Q04864

Immunogen: This C-rel (NFkB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 411~440 amino acids from the center region of human C-rel(NFkB).

Isotype: Ig

Description: Other names: C-Rel proto-oncogene protein, C-Rel protein, REL,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This C-rel (NFkB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 411~440 amino acids from the center region of human C-rel(NFkB).

Molecular Weight: 68520 DA

Comments:

Background: Nuclear factor (NF)-kappa B is a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF-kB regulates the expression of a wide variety of genes that involved in apoptosis, viral life cycle, tumorigenesis, autoimmune diseases and inflammation. NF-kB is a heterodimer of members of the rel family of proteins such as p50, p65, and c-rel. In most cells, inhibitory IkB proteins sequester NF-kB/Rel in the cytoplasm. Cellular stimulation precipitates degradation of IkB and modification of NF-kB/Rel proteins, permitting translocation of NF-kB/Rel (c-Rel and RelA) to the nucleus for action on target genes. The important role of c-Rel in B-cell development, growth, and survival has been intensively studied, as well as its function in differentiation and lymphopoiesis (particularly lymphoid cancer).

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-C-rel (NFkB) Pab (ABIN390314)in Jurkat (left) and Hela (right) cell line lysate. C-rel (NFkB)(arrow) was detected using the purified Pab.

Publications

Product: Bernard, Gosselin, Monte et al.: "Involvement of Rel/nuclear factor-kappaB transcription factors in keratinocyte senescence." in: Cancer research, Vol. 64, Issue 2, pp. 472-81, 2004 (PubMed).

Phelps, Ghosh: "Discreet mutations from c-Rel to v-Rel alter kappaB DNA recognition, IkappaBalpha binding, and dimerization: implications for v-Rel oncogenicity." in: Oncogene, Vol. 23, Issue 6, pp. 1229-38, 2004 (PubMed).

Jain, Ma, Lopez-Granados et al.: "Specific NEMO mutations impair CD40-mediated c-Rel activation and B cell terminal differentiation." in: The Journal of clinical investigation, Vol. 114, Issue 11, pp. 1593-602, 2004 (PubMed).