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C-rel (NfkB) (C-Term, Asp504) antibody
Western Blotting (WB), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This C-rel (NFkB) (C-term D504) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499~528 amino acids from the C-terminal region of human C-rel(NFkB).|
|Description||Other names: C-Rel proto-oncogene protein, C-Rel protein, REL,|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This C-rel (NFkB) (C-term D504) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499~528 amino acids from the C-terminal region of human C-rel(NFkB).|
|Molecular Weight||68520 DA|
Background: Nuclear factor (NF)-kappa B is a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF-kB regulates the expression of a wide variety of genes that involved in apoptosis, viral life cycle, tumorigenesis, autoimmune diseases and inflammation. NF-kB is a heterodimer of members of the rel family of proteins such as p50, p65, and c-rel. In most cells, inhibitory IkB proteins sequester NF-kB/Rel in the cytoplasm. Cellular stimulation precipitates degradation of IkB and modification of NF-kB/Rel proteins, permitting translocation of NF-kB/Rel (c-Rel and RelA) to the nucleus for action on target genes. The important role of c-Rel in B-cell development, growth, and survival has been intensively studied, as well as its function in differentiation and lymphopoiesis (particularly lymphoid cancer).
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Signaling, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of anti-C-rel (NFkB) Pab (ABIN390315)in Jurkat (left) and Hela (right) cell line lysate. C-rel (NFkB)(arrow) was detected using the purified Pab.|
Bernard, Gosselin, Monte et al.: "Involvement of Rel/nuclear factor-kappaB transcription factors in keratinocyte senescence." in: Cancer research, Vol. 64, Issue 2, pp. 472-81, 2004 (PubMed).
Phelps, Ghosh: "Discreet mutations from c-Rel to v-Rel alter kappaB DNA recognition, IkappaBalpha binding, and dimerization: implications for v-Rel oncogenicity." in: Oncogene, Vol. 23, Issue 6, pp. 1229-38, 2004 (PubMed).
Jain, Ma, Lopez-Granados et al.: "Specific NEMO mutations impair CD40-mediated c-Rel activation and B cell terminal differentiation." in: The Journal of clinical investigation, Vol. 114, Issue 11, pp. 1593-602, 2004 (PubMed).