Interleukin 1 Receptor, Type I (IL1R1) (C-Term,Glu487) antibody

Antigen: Interleukin 1 Receptor, Type I (IL1R1)

Binding Site: C-Term,Glu487

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390328

Additional Information

Alternative name: IL1R

Gene ID: 8808

UniProt: Q9HB29

Immunogen: This IL1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal(E487) region of human IL1R.

Isotype: Ig

Description: Other names: Interleukin-1 receptor-like 2 [Precursor], IL-1Rrp2, Interleukin-1 receptor-related protein 2, IL1R-rp2, IL1RL2, IL1RRP2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IL1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal(E487) region of human IL1R.

Molecular Weight: 65405 DA

Comments:

Background: IL1R is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. IL1R is a receptor for interleukin 1 family member 9 (IL1F9). Binding to the agonist leads to the activation of NF-kappa-B. The gene for this protein and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Application Details

Application Notes: The suggested dilution is: ELISA1:1,000 Western blotting 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-IL1R (ABIN390328) in HL60 cell line lysates (35ug/lane). IL1R (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with IL1R antibody (C-term E487), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Lovenberg, Crowe, Liu et al.: "Cloning of a cDNA encoding a novel interleukin-1 receptor related protein (IL 1R-rp2)." in: Journal of neuroimmunology, Vol. 70, Issue 2, pp. 113-22, 1996 (PubMed).

Torigoe, Ushio, Okura et al.: "Purification and characterization of the human interleukin-18 receptor." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25737-42, 1997 (PubMed).

Dale, Nicklin: "Interleukin-1 receptor cluster: gene organization of IL1R2, IL1R1, IL1RL2 (IL-1Rrp2), IL1RL1 (T1/ST2), and IL18R1 (IL-1Rrp) on human chromosome 2q." in: Genomics, Vol. 57, Issue 1, pp. 177-9, 1999 (PubMed).

Debets, Timans, Homey et al.: "Two novel IL-1 family members, IL-1 delta and IL-1 epsilon, function as an antagonist and agonist of NF-kappa B activation through the orphan IL-1 receptor-related protein 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1440-6, 2001 (PubMed).