Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1) (Center) antibody

Antigen: Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1)

Synonyms: ICE, P45, IL1BC, Il1bc, Ice, DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370, CASP1, LOC100033888

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390657

Additional Information

Alternative name: CASP1

Gene ID: 834

UniProt: P29466

Immunogen: This CASP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300~330 amino acids from the Center region of human CASP1.

Isotype: Ig

Description: Other names: Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, IL-1 beta-converting enzyme, ICE, p45, Caspase-1 subunit p20, Caspase-1 subunit p10, IL1BCE, IL1BC, CASP1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CASP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300~330 amino acids from the Center region of human CASP1.

Molecular Weight: 45159 DA

Comments:

Background: CASP1 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP1 was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This protein has been shown to induce cell apoptosis and may function in various developmental stages.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of CASP1 antibody (Center) (ABIN390657) in HL60 cell line lysates (35ug/lane). CASP1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung tissue with CASP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-231 cells using CASP1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Basak, Pathak, Bhattacharyya et al.: "NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4279-88, 2005 (PubMed).

Nasirudeen, Liu: "Gene expression profiling by microarray analysis reveals an important role for caspase-1 in dengue virus-induced p53-mediated apoptosis." in: Journal of medical virology, Vol. 81, Issue 6, pp. 1069-81, 2009 (PubMed).