FK506 Binding Protein 5 (FKBP5) (N-Term) antibody

Antigen: FK506 Binding Protein 5 (FKBP5)

Synonyms: P54, AIG6, FKBP51, FKBP54, PPIase, Ptg-10, MGC111006, Dit1, 51kDa, D17Ertd592e, zgc:64082, wu:fc31g11, wu:fl87b03, si:zc263a23.8, FKBP5, DKFZp469O2021

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390691

Additional Information

Alternative name: FKBP5

Gene ID: 2289

UniProt: Q13451

Immunogen: This FKBP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminal region of human FKBP5.

Isotype: Ig

Description: Other names: FK506-binding protein 5, Peptidyl-prolyl cis-trans isomerase, PPIase, Rotamase, 51 kDa FK506-binding protein, FKBP-51, 54 kDa progesterone receptor-associated immunophilin, FKBP54, P54, FF1 antigen, HSP90-binding immunophilin, Androgen-regulated protein 6

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FKBP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminal region of human FKBP5.

Molecular Weight: 51212 DA

Comments:

Background: The protein is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of FKBP5 Antibody (N-term) (ABIN390691) in K562,293 cell line lysates (35ug/lane). FKBP5 (arrow) was detected using the purified Pab.

FKBP5 Antibody (N-term) (ABIN390691) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FKBP5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

FKBP5 Antibody (N-term) (ABIN390691) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Pei, Li, Fridley et al.: "FKBP51 affects cancer cell response to chemotherapy by negatively regulating Akt." in: Cancer cell, Vol. 16, Issue 3, pp. 259-66, 2009 (PubMed).