Junctional Adhesion Molecule 3 (JAM3) (C-Term) antibody

Antigen: Junctional Adhesion Molecule 3 (JAM3)

Synonyms: JAMC, JAM-C, FLJ14529, JAM-3, Jcam3, C85515, 1110002N23Rik, MGC94974, JAM3, MGC140154, fb30h11, fc08c06, fc13e04, fc25g11, sr:nyz155, wu:fb30h11, wu:fc08c06, wu:fc13e04, wu:fc25g11, jamc.2

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390732

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: JAM3 (C-term)

Gene ID: 83700

Swiss-Prot: Q9BX67

Immunogen: This JAM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281~310 amino acids from the C-terminal region of human JAM3.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Junctional adhesion molecule C, JAM-C, Junctional adhesion molecule 3, JAM-3, JAM-2 , UNQ859/PRO1868, JAM3

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human JAM3.

Molecular Weight: 35020 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of JAM3 Antibody (C-term) (ABIN390732) in Y79 cell line lysates (35ug/lane). JAM3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with JAM3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

JAM3 Antibody (C-term) (ABIN390732) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.