Bloom Syndrome, RecQ Helicase-Like (BLM) (C-Term) antibody

Antigen: Bloom Syndrome, RecQ Helicase-Like (BLM)

Synonyms: BS, RECQ2, RECQL2, RECQL3, MGC126616, MGC131618, MGC131620, BLM, blm-A, xBLM, ORF1

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390751

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: BLM (C-term)

Gene ID: 641

Swiss-Prot: P54132

Immunogen: This BLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1316~1346 amino acids from the C-terminal region of human BLM.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Bloom syndrome protein, RecQ protein-like 3, DNA helicase, RecQ-like type 2, RECQL3, RECQ2, BLM

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human BLM.

Molecular Weight: 159000 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of BLM Antibody (C-term) (ABIN390751) in Jurkat cell line lysates (35ug/lane). BLM (arrow) was detected using the purified Pab.

BLM Antibody (C-term) (ABIN390751) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

BLM Antibody (C-term) (ABIN390751) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.