HLA Class II DR alpha (HLA-DRA) (N-Term) antibody

Antigen: HLA Class II DR alpha (HLA-DRA)

Synonyms: HLA-DRA1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390770

Additional Information

Alternative name: HLA-DRA

Gene ID: 3122

UniProt: P01903

Immunogen: This HLA-DRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54~84 amino acids from the N-terminal region of human HLA-DRA .

Isotype: Ig

Description: Other names: HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, HLA-DRA1, HLA-DRA

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HLA-DRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54~84 amino acids from the N-terminal region of human HLA-DRA .

Molecular Weight: 28607 DA

Comments:

Background: HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of HLA-DRA Antibody (N-term) (ABIN390770) in Ramos cell line lysates (35ug/lane). HLA-DRA (arrow) was detected using the purified Pab.

HLA-DRA Antibody (N-term) (ABIN390770) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HLA-DRA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HLA-DRA Antibody (N-term) (ABIN390770) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: De Jager, Jia, Wang et al.: "Meta-analysis of genome scans and replication identify CD6, IRF8 and TNFRSF1A as new multiple sclerosis susceptibility loci." in: Nature genetics, Vol. 41, Issue 7, pp. 776-82, 2009 (PubMed).