CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB) (N-Term) antibody

Antigen: CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB)

Synonyms: LAP, CRP2, TCF5, IL6DBP, NF-IL6, MGC32080, C/EBP-beta, NF-M, Nfil6, IL-6DBP, C/EBPbeta, Il6dbp, CEBPB, C/EBP beta, lap, crp2, tcf5, il6dbp, nf-il6, MGC107842, c/ebp-beta, xC/EBPbeta, cebpbb, cb380, c/ebpb, fe11e05, wu:fe11e05, LOC100136165

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390791

Additional Information

Alternative name: CEBPB

Gene ID: 1051

UniProt: P17676

Immunogen: This CEBPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56~85 amino acids from the N-terminal region of human CEBPB.

Isotype: Ig

Description: Other names: CCAAT/enhancer-binding protein beta, C/EBP beta, Nuclear factor NF-IL6, Transcription factor 5, PP9092, TCF5, CEBPB

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CEBPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56~85 amino acids from the N-terminal region of human CEBPB.

Molecular Weight: 36106 DA

Comments:

Background: CEBPB is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. This protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, It can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.

Synonyms: LAP, CRP2, TCF5, IL6DBP, NF-IL6, MGC32080, C/EBP-beta, NF-M, Nfil6, IL-6DBP, C/EBPbeta, Il6dbp, CEBPB, C/EBP beta, lap, crp2, tcf5, il6dbp, nf-il6, MGC107842, c/ebp-beta, xC/EBPbeta, cebpbb, cb380, c/ebpb, fe11e05, wu:fe11e05, LOC100136165

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of CEBPB Antibody (N-term) (ABIN390791) in Hela cell line lysates (35ug/lane). CEBPB (arrow) was detected using the purified Pab.

Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (N-term) (ABIN390791). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2) .

Flow cytometric analysis of hela cells using CEBPB Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Buck, Poli, van der Geer et al.: "Phosphorylation of rat serine 105 or mouse threonine 217 in C/EBP beta is required for hepatocyte proliferation induced by TGF alpha." in: Molecular cell, Vol. 4, Issue 6, pp. 1087-92, 2000 (PubMed).