RAN, Member RAS Oncogene Family (RAN) (Center) antibody

Antigen: RAN, Member RAS Oncogene Family (RAN)

Synonyms: TC4, Gsp1, ARA24, ran-1, ran, MGC53579, RAN, tc4, gsp1, ara24, MGC69330, MGC127753, DKFZp459G1118, DKFZp468B1019, xran

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390805

Additional Information

Alternative name: RAN

Gene ID: 5901

UniProt: P62826

Immunogen: This RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114~144 amino acids from the Center region of human RAN.

Isotype: Ig

Description: Other names: GTP-binding nuclear protein Ran, GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24, OK/SW-cl.81, ARA24, RAN

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114~144 amino acids from the Center region of human RAN.

Molecular Weight: 24423 DA

Comments:

Background: RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RAN Antibody (Center) (ABIN390805) in mouse testis tissue lysates (35ug/lane). RAN (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis reacted with RAN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of K562 cells using RAN Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).