Aurora Kinase A (AURKA) (C-Term) antibody

Antigen: Aurora Kinase A (AURKA)

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390849

Additional Information

Alternative name: AURKA

Gene ID: 6790

UniProt: O14965

Immunogen: This AURKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335~365 amino acids from the C-terminal region of human AURKA.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase 6, Aurora kinase A, Aurora-A, Serine/threonine kinase 15, Aurora/IPL1-related kinase 1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, STK6, STK15, BTAK, AURA, ARK1, AIK, AURKA

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AURKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335~365 amino acids from the C-terminal region of human AURKA.

Molecular Weight: 45809 DA

Comments:

Background: AIK is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. This protein is found at the centrosome in interphase cells and at the spindle poles in mitosis.

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of AURKA Antibody (C-term) (ABIN390849) in MCF-7 cell line lysates (35ug/lane). AURKA (arrow) was detected using the purified Pab.

Western blot analysis of AURKA (arrow) using rabbit polyclonal AURKA Antibody (C-term) (ABIN390849). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKA gene (Lane 2) (Origene Technologies).

Publications

Product: Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).