GTPase Activating Protein and VPS9 Domains 1 (GAPVD1) (C-Term) antibody

Antigen: GTPase Activating Protein and VPS9 Domains 1 (GAPVD1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390858

Additional Information

Alternative name: GAPVD1

Gene ID: 26130

UniProt: Q14C86

Immunogen: This GAPVD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1045~1075 amino acids from the C-terminal region of human GAPVD1.

Isotype: Ig

Description: Other names: GTPase-activating protein and VPS9 domain-containing protein 1, Rab5-activating protein 6, GAPex-5, RAP6, KIAA1521, GAPEX5, GAPVD1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GAPVD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1045~1075 amino acids from the C-terminal region of human GAPVD1.

Molecular Weight: 164980 DA

Comments:

Background: GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

Western blot analysis of GAPVD1 Antibody (C-term) (ABIN390858) in 293 cell line lysates (35ug/lane). GAPVD1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with GAPVD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

GAPVD1 Antibody (C-term) (ABIN390858) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).