Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/enoyl-Coenzyme A Hydratase (Trifunctional Protein), alpha Subunit (HADHA) (C-Term) antibody

Antigen: Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/enoyl-Coenzyme A Hydratase (Trifunctional Protein), alpha Subunit (HADHA)

Synonyms: GBP, ECHA, HADH, MTPA, LCHAD, MGC1728, TP-ALPHA, Mtpa, C77020, MGC105338, RGD1560655, MGC128917, MGC76057, HADHA, MGC82638, cb1055, im:7136067, wu:fa14c08, DKFZp468A155

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390869

Additional Information

Alternative name: HADHA

Gene ID: 3030

UniProt: P40939

Immunogen: This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 734~763 amino acids from the C-terminal region of human HADHA.

Isotype: Ig

Description: Other names: Trifunctional enzyme subunit alpha, mitochondrial, TP-alpha, 78 kDa gastrin-binding protein, Long-chain enoyl-CoA hydratase, Long chain 3-hydroxyacyl-CoA dehydrogenase, HADH, HADHA

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 734~763 amino acids from the C-terminal region of human HADHA.

Molecular Weight: 83000 DA

Comments:

Background: HADHA is the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

Western blot analysis of HADHA Antibody (C-term) (ABIN390869) in A2058, A375, NCI-H460, Romas cell line lysates (35ug/lane). HADHA (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

HADHA Antibody (C-term) (ABIN390869) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Sims, Brackett, Powell et al.: "The molecular basis of pediatric long chain 3-hydroxyacyl-CoA dehydrogenase deficiency associated with maternal acute fatty liver of pregnancy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 3, pp. 841-5, 1995 (PubMed).