CREB Regulated Transcription Coactivator 1 (CRTC1) (N-Term) antibody

Antigen: CREB Regulated Transcription Coactivator 1 (CRTC1)

Synonyms: MECT1, TORC1, WAMTP1, FLJ14027, KIAA0616, Mect1, R74955, AI413414, MGC90786, mKIAA0616, CRTC1, Crtc1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390956

Additional Information

Alternative name: TORC1

Gene ID: 23373

UniProt: Q6UUV9

Immunogen: This TORC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90~119 amino acids from the N-terminal region of human TORC1.

Isotype: Ig

Description: Other names: CREB-regulated transcription coactivator 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1, Mucoepidermoid carcinoma translocated protein 1, WAMTP1, TORC1, MECT1, KIAA0616, CRTC1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TORC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90~119 amino acids from the N-terminal region of human TORC1.

Molecular Weight: 67300 DA

Comments:

Background: TORC1 is the transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation and Enhances the interaction of CREB1 with TAF4. It regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of TORC1 Antibody (N-term) (ABIN390956) in NCI-H460 cell line lysates (35ug/lane). TORC1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue with TORC1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using TORC1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Grimwood, Gordon, Olsen et al.: "The DNA sequence and biology of human chromosome 19." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).