MAP-EB3 (Center) antibody

Antigen: MAP-EB3

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390961

Additional Information

Gene ID: 22924

UniProt: Q9UPY8

Immunogen: This MAP-EB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210~240 amino acids from the Center region of human MAP-EB3.

Isotype: Ig

Description: Other names: Microtubule-associated protein RP/EB family member 3, End-binding protein 3, EB3, EB1 protein family member 3, EBF3, RP3, MAPRE3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAP-EB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210~240 amino acids from the Center region of human MAP-EB3.

Molecular Weight: 31982 DA

Comments:

Background: MAP-EB3 may be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. It may play a role in cell migration.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of MAP-EB3 Antibody (Center) (ABIN390961) in mouse brain tissue lysates (35ug/lane). MAP-EB3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle with MAP-EB3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Yeh, Chuang, Sung: "Dynein light chain rp3 acts as a nuclear matrix-associated transcriptional modulator in a dynein-independent pathway." in: Journal of cell science, Vol. 118, Issue Pt 15, pp. 3431-43, 2005 (PubMed).