|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Aurora Kinase C (AURKC) (C-Term) antibody
|Synonyms||AIE2, AIK3, AurC, STK13, aurora-C, AIE1, Stk13, AURKC|
Alternatives Western Blotting (WB), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Aurora-C.|
|Description||Other names: Aurora/Ipl1/Eg2 protein 2, Serine/threonine protein kinase 13, Aurora/Ipl1-related kinase 3, Aurora-C|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Aurora-C.|
|Molecular Weight||35591 DA|
Background: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
|The anti-Aurora-C C-term Pab (ABIN390975) is used in Western blot to detect Aurora C in A375 cell lysate.|
Bernard, Sanseau, Henry et al.: "Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter." in: Genomics, Vol. 53, Issue 3, pp. 406-9, 1998 (PubMed).
Tseng, Chen, Hsu et al.: "Protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (AIE1, AIE2) related to yeast and fly chromosome segregation regulators." in: DNA and cell biology, Vol. 17, Issue 10, pp. 823-33, 1998 (PubMed).
Kimura, Matsuda, Yoshioka et al.: "Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7334-40, 1999 (PubMed).
|Hosts||Rabbit (42), Goat (3), Mouse (3)|
|Reactivities||Human (46), Mouse (Murine) (20), Cow (Bovine) (19), Pig (Porcine) (19), Rat (Rattus) (19), Horse (Equine) (18), Dog (Canine) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (31), Immunofluorescence (IF) (27), ELISA (23), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (IHC) (2), Dot Blot (DB) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (7), C-Term (3), Internal Region (3), Transcript Variant 1 (3), AA 5-20 (1), Center (1), N-Term,Gly11 (1), N-Term,Met1 (1)|