Aurora Kinase A (AURKA) (N-Term,Ser89) antibody

Antigen: Aurora Kinase A (AURKA)

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Binding Site: N-Term,Ser89

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390982

Additional Information

Alternative name: Aurora-A

Gene ID: 6790

UniProt: O14965

Immunogen: This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Aurora-A.

Isotype: Ig

Description: Other names: AUR2, Aurora/IPL1-related kinase 1, Aurora-related kinase 1, hARK1, Aurora-A, Breast-tumor-amplified kinase, Serine/threonine kinase 6

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Aurora-A.

Molecular Weight: 45809 DA

Comments:

Background: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy.

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Aurora-A (ABIN390982) in A2058 cell line lysate (35ug/lane).Aurora-A (arrow) was detected using the purified Pab (1:25 dilution).

Western blot analysis of Aurora-A (arrow) using rabbit polyclonal Aurora-A N-term Antibody (ABIN390982). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Aurora-A gene (Lane 2).

Publications

Product: Shindo, Nakano, Kuroyanagi et al.: "cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2." in: Biochemical and biophysical research communications, Vol. 244, Issue 1, pp. 285-92, 1998 (PubMed).

Nigg: "Mitotic kinases as regulators of cell division and its checkpoints." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 1, pp. 21-32, 2001 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Tanaka, Ueda, Kanamori et al.: "Cell-cycle-dependent regulation of human aurora A transcription is mediated by periodic repression of E4TF1." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10719-26, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).