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Protein Kinase D3 (PRKD3) (AA 860-890), (C-Term) antibody

Details for Product No. ABIN391015, Supplier: Log in to see
Antigen
  • PRKD3
  • EPK2
  • PKC-NU
  • PKD3
  • PRKCN
  • nPKC-NU
  • 4930557O20Rik
  • 5730497N19Rik
  • Pkcnu
  • Prkcn
Epitope
AA 860-890, C-Term
22
16
15
14
13
13
8
7
4
3
2
2
2
1
1
Reactivity
Human
100
40
14
Host
Rabbit
100
7
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
8
8
8
6
6
6
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
78
51
50
22
20
15
13
3
1
Supplier
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Immunogen This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 860-890 AA from the C-terminal region of human PKC nu.
Clone RB1290
Isotype Ig
Specificity This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PKC nu (PRKD3 Antibody Abstract)
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
Synonyms: Protein kinase C, nu type, nPKC-nu, Protein kinase EPK2, PKD3
Molecular Weight 100470 Da
Gene ID 23683
UniProt O94806
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.71 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase D3 (PRKD3) (AA 860-890), (C-Term) antibody (ABIN391015) Western blot analysis of anti-PKCnu C-term Pab (ABIN391015) in NCI-H460 cell lysate. ...
Western Blotting (WB) image for anti-Protein Kinase D3 (PRKD3) (AA 860-890), (C-Term) antibody (ABIN391015) Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells b...
Immunohistochemistry (IHC) image for anti-Protein Kinase D3 (PRKD3) (AA 860-890), (C-Term) antibody (ABIN391015) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Yeaman, Ayala, Wright et al.: "Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network." in: Nature cell biology, Vol. 6, Issue 2, pp. 106-12, 2004 (PubMed).

Rey, Yuan, Young et al.: "Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23773-85, 2003 (PubMed).

Matthews, Dayalu, Thompson et al.: "Regulation of protein kinase Cnu by the B-cell antigen receptor." in: The Journal of biological chemistry, Vol. 278, Issue 11, pp. 9086-91, 2003 (PubMed).