Western blot analysis of anti-PKCnu C-term Pab (ABIN391015) in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells. lanes 2 and 6: GFP-PKD-transfected cells. lanes 3 and 7: GFP-PKD2-transfected cells. lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (ABIN391014) and C-term (ABIN391015) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using ABIN391014 and ABIN391015 antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma